CD63 Antibody / LAMP-3

SKU: orb749918

Description

This mAb recognizes protein of 26kDa-60kDa, which is identified as CD63/LAMP-3. Its epitope is different from that of mAb LAMP3/529. The tetraspanins are integral membrane proteins expressed on cell surface and granular membranes of hematopoietic cells and are components of multi-molecular complexes with specific integrins. The tetraspanin CD63/LAMP-3 is a lysosomal membrane glycoprotein that translocates to the plasma membrane after platelet activation. It is expressed on activated platelets, monocytes and macrophages, and is weakly expressed on granulocytes, T cell and B cells. It is located on the basophilic granule membranes and on the plasma membranes of lymphocytes and granulocytes. CD63 is a member of the TM4 superfamily of leukocyte glycoproteins that includes CD9, CD37 and CD53, which contain four transmembrane regions. CD63/LAMP-3 may play a role in phagocytic and intracellular lysosome-phagosome fusion events. Deficiency is associated with Hermansky-Pudlak syndrome and is strongly expressed during the early stages of melanoma progression.

Images & Validation

• Tested Applications: FACS, IHC-P
• Dilution range: Flow cytometry: 1-2ug/million cells,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
• Reactivity: Human
• Application Notes:
  Optimal dilution of the LAMP-3 antibody should be determined by the researcher.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgG2a, kappa
• Clone No.: LAMP3/968
• Immunogen: Recombinant human full-length protein was used as the immunogen for the CD63 / LAMP-3 antibody.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Disclaimer: For research use only

Flow cytometry testing of human MCF7 cells with LAMP-3 antibody (clone LAMP3/968); Gray = unstained, Orange = CF555-labeled LAMP-3 antibody.

FACS staining of bead-bound exosomes derived from human MCF7 cells: Gray = unstained, Orange = CF555-labeled LAMP-3 antibody.

Flow cytometry testing of PFA-fixed human U-87 MG cells with LAMP-3 antibody (clone LAMP3/968); Red = isotype control, Blue = LAMP-3 antibody.

IHC: Formalin-fixed, paraffin-embedded human prostate carcinoma stained with CD63 / LAMP-3 antibody (LAMP3/968).

IHC: Formalin-fixed, paraffin-embedded human melanoma stained with CD63 / LAMP-3 antibody (LAMP3/968).

SDS-PAGE Analysis of Purified, BSA-Free LAMP-3 Antibody (clone LAMP3/968). Confirmation of Integrity and Purity of the Antibody.

Quick Database Links

UniProt

P08962