CD63 Antibody / LAMP-3

• Catalog Number: orb606864
• Category: Antibodies
• Description: This mAb recognizes protein of 26kDa-60kDa, which is identified as CD63/LAMP-3. The tetraspanins are integral membrane proteins expressed on cell surface and granular membranes of hematopoietic cells and are components of multi-molecular complexes with specific integrins. The tetraspanin CD63/LAMP-3 is a lysosomal membrane glycoprotein that translocates to the plasma membrane after platelet activation. It is expressed on activated platelets, monocytes and macrophages, and is weakly expressed on granulocytes, T cell and B cells. It is located on the basophilic granule membranes and on the plasma membranes of lymphocytes and granulocytes. CD63 is a member of the TM4 superfamily of leukocyte glycoproteins that includes CD9, CD37 and CD53, which contain four transmembrane regions. CD63/LAMP-3 may play a role in phagocytic and intracellular lysosome-phagosome fusion events. Deficiency is associated with Hermansky-Pudlak syndrome and is strongly expressed during the early stages of melanoma progression.
• Clonality: Recombinant
• Species/Host: Rabbit
• Isotype: Rabbit IgG, kappa
• Conjugation: Unconjugated
• Reactivity: Human
• Immunogen: Recombinant human full-length protein was used as the immunogen for the recombinant CD63 antibody.
• UniProt ID: P08962
• Tested applications: FACS, IHC-P
• Dilution range: Flow cytometry: 1-2ug/million cells,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
• Application notes: Optimal dilution of the recombinant CD63 antibody should be determined by the researcher.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Antibody Type: Primary Antibody
• Clone Number: LAMP3/2990R
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Research Area: Epigenetics
• Note: For research use only

IHC staining of FFPE human prostate carcinoma with recombinant CD63 antibody (clone LAMP3/2990R). HIER: boil tissue sections in 10 mM Tris with 1 mM EDTA, pH9 for 10-20 min followed by cooling.

Flow cytometry testing of PFA-fixed human U-87 MG cells with CD63 antibody (clone LAMP3/2990R); Red = isotype control, Blue = CD63 antibody.

SDS-PAGE analysis of purified, BSA-free recombinant CD63 antibody (clone LAMP3/2990R) as confirmation of integrity and purity.