SETD2 Antibody

SKU: orb412165

Description

The SETD2 Antibody is suitable for IF, IP, WB. It is a Polyclonal, Unconjugated antibody which raised against Recombinant fusion protein of human SETD2 .Purification: The antibody was purified by immunogen affinity chromatography.

Images & Validation

• Tested Applications: IF, IP, WB
• Dilution range: WB: 1:500-2000, IF/ICC: 1:50-200, IP: 1:50-100
• Reactivity: Human, Mouse, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Recombinant fusion protein of human SETD2
• Purification: The antibody was purified by immunogen affinity chromatography.

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Disclaimer: For research use only

Alternative Names

HIF1; HYPB; KIAA1732; KMT3A; SET2; Histone-lysine N-methyltransferase SETD2; HIF-1; Huntingtin yeast partner B; Huntingtin-interacting protein 1; HIP-1; Huntingtin-interacting protein B; Lysine N-methyltransferase 3A; SET domain-containing protein 2; hSET2; p231HBP

Western blot analysis of SETD2 expression in mouse brain (A), mouse lung (B) whole cell lysates. (Predicted band size: 175; 192; 287 kD; Observed band size: 288 kD)

Immunofluorescent analysis of SETD2 staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

Quick Database Links

UniProt

E9Q5F9, Q9BYW2

NCBI Gene ID

29072, 235626