Anti-Hu CD3 Purified

• Catalog Number: orb44337
• Category: Antibodies
• Description: Mouse monoclonal antibody to CD3
• Clonality: Monoclonal
• Clone Number: MEM-57
• Tested applications: FC, IP
• Reactivity: Human
• Isotype: Mouse IgG2a kappa
• Immunogen: Human thymocytes and T lymphocytes.
• Antibody Type: Primary Antibody
• Concentration: 1 mg/ml
• Purity: Purified by protein-A affinity chromatography.
• Conjugation: Unconjugated
• Target: CD3
• Entrez: 916
• UniProt ID: P07766
• RRID: AB_10999126
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Alternative names: anti CD 3 antibody, anti Cd247 antibody, anti CD24
• Note: For research use only
• Application notes: Immunoprecipitation: The antibody MEM-57 immunoprecipitates from a detergent lysate of surface-radioiodinated T cells a strong zone of about 22 kDa and a weak 28-kDa zone, which is typical pattern yielded by a reference antibody Leu-4 (SK7). Flow cytometry: Recommended dilution: 2 - 5 μg/ml; positive control: peripheral blood lymphocytes, JURKAT human leukemia T cell line.
• Expiration Date: 12 months from date of receipt.

Mass cytometry analysis (surface staining) of PBMC after Ficoll-Paque separation with anti-human CD3 (MEM-57) Dy161. Gated on singlets.

Separation of human CD3 positive lymphocytes (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD3 (MEM-57) purified antibody (concentration in sample 0.33 µg/ml) GAM APC.

Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD3 (MEM-57) purified antibody (concentration in sample 0.33 µg/ml) GAM APC.