Anti-Hu CD3 Purified Low Endotoxin

• Catalog Number: orb44338
• Category: Antibodies
• Description: Mouse monoclonal antibody to CD3
• Clonality: Monoclonal
• Clone Number: MEM-57
• Tested applications: FA, FC, IP
• Reactivity: Human
• Isotype: Mouse IgG2a kappa
• Immunogen: Human thymocytes and T lymphocytes.
• Antibody Type: Primary Antibody
• Concentration: 1 mg/ml
• Purity: Purified by protein-A affinity chromatography.
• Conjugation: Unconjugated
• Target: CD3
• Entrez: 916
• UniProt ID: P07766
• RRID: AB_10997861
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Phosphate buffered saline (PBS), pH 7.4
• Alternative names: anti CD 3 antibody, anti Cd247 antibody, anti CD24
• Note: For research use only
• Application notes: Low Endotoxin, Azide free format. Product does not contain preservatives and is intended primarily for functional applications. Preservative containing buffers are available for IHC applications
• Expiration Date: 12 months from date of receipt.

Mass cytometry analysis (surface staining) of PBMC after Ficoll-Paque separation with anti-human CD3 (MEM-57) Dy161. Gated on singlets.

Separation of human CD3 positive lymphocytes (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD3 (MEM-57) purified antibody (low endotoxin, concentration in sample 0.33 μg/ml) GAM APC.

Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD3 (MEM-57) purified antibody (low endotoxin, concentration in sample 0.33 μg/ml) GAM APC.