Adipophilin Antibody / Perilipin 2 / ADRP / PLIN2

SKU: orb385951

Description

Adipophilin, also called Perilipin 2, ADRP (Adipose differentiation related protein), and PLIN2, belongs to the perilipin family, members of which coat intracellular lipid storage droplets. This protein is associated with the lipid globule surface membrane material and maybe involved in development and maintenance of adipose tissue. It is not restricted to adipocytes as previously thought, but is found in a wide range of cultured cell lines, including fibroblasts, endothelial and epithelial cells, and tissues, such as lactating mammary gland, adrenal cortex, Sertoli and Leydig cells, and hepatocytes in alcoholic liver cirrhosis, suggesting that it may serve as a marker of lipid accumulation in diverse cell types and diseases.

Images & Validation

• Tested Applications: ELISA, IHC-P
• Dilution range: ELISA (order BSA/sodium azide-free format for coating),Immunohistochemistry (FFPE): 2-4ug/ml for 30 min at RT
• Reactivity: Human
• Application Notes:
  Titering of the PLIN2 antibody may be required for optimal performance.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgG2b, kappa
• Clone No.: ADFP/1494
• Immunogen: A human partial recombinant protein was used as the immunogen for this ADRP antibody.

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Disclaimer: For research use only

IHC testing of FFPE human adrenal tissue with ADRP antibody (clone ADFP/1494). Required HIER: steam sections in pH6, 10mM citrate buffer for 10-20 min.