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Catalog Number | orb626873 |
---|---|
Category | Antibodies |
Description | Rabbit polyclonal antibody to F4/80 |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | ELISA, IF, IHC, WB |
Reactivity | Human, Mouse, Rat |
Isotype | IgG |
Immunogen | EGF-like module containing, mucin-like, hormone receptor-like sequence 1 |
Antibody Type | Primary Antibody |
Dilution range | WB: 1:500-1:1000; IHC: 1:20-1:200; IF: 1:50-1:500 |
Form/Appearance | liquid |
Purity | ≥95% as determined by SDS-PAGE |
Conjugation | Unconjugated |
MW | 130-140 kDa |
Target | ADGRE1 |
UniProt ID | Q14246 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
Immunofluorescent analysis of (10% Formaldehyde) fixed Raw264.7 cells using orb626873 (F4/80 antibody) at dilution of 1:50 and FITC-conjugated Goat Anti-Rabbit IgG (H+L).
HL-60 cells were subjected to SDS PAGE followed by western blot with orb626873 (F4/80 Antibody) at dilution of 1:1000.
Immunohistochemistry of paraffin-embedded mouse spleen tissue slide using orb626873 (F4/80 Antibody) at dilution of 1:200.
IF, IHC-Fr, IHC-P | |
Guinea pig, Porcine, Rat | |
Mouse | |
Rabbit | |
Polyclonal | |
Unconjugated |
WB | |
Mouse, Rat | |
Human | |
Mouse | |
Monoclonal | |
Unconjugated |