5-Propargylamino-dCTP - Solution

• Catalog Number: orb1733847
• Category: Small Molecules
• Description: Propargylamino-dCTP is recommended for two-step labeling of DNA e.g. by PCR. It is enzymatically incorporated into DNA as substitute for its natural counterpart dCTP. The resulting Amine-functionalized DNA can subsequently be labeled via the classic Amine/NHS Ester reaction that offers the choice: to introduce a Biotin group (via NHS Ester of Biotin) for subse-quent purification tasks; to introduce fluorescent group (via NHS Ester of fluorescent dyes) for subsequent microscopic imaging.
• Form/Appearance: solution in water; Color: slightly yellow; pH: 7.5 ± 0.5
• Concentration: 100 mM-110 mM
• Purity: ≥ 93% (HPLC)
• MW: Theoretical MW: 520.22 g/mol (free acid); Detected MW: 520.02 g/mol (free acid)
• Application notes: Incorporation into DNA by PCR with Taqpolymerasein-house data. ; Spectroscopic Propertie: λmax 294 nm, ε 9.3 L mmol-1 cm-1 (Tris-HCl, pH 7.5).
• CAS Number: 115899-39-3
• Formula: C₁₂H₁₉N₄O₁₃P₃
• SMILES: NCC#Cc1cn(c(=O)nc1N)[C@@H]1O[C@H](COP(=O)(O)OP(=O)(O)OP(=O)(O)O)[C@@H](O)C1
• Storage: store at -20 °C. Short term exposure (up to 1 week cumulative) to ambient temperature possible
• Note: For research use only