Human Transcription activator BRG1 (SMARCA4) ELISA Kit

• Catalog Number: orb2668249
• Category: Assays and Kits
• Description: Human Transcription activator BRG1 (SMARCA4) ELISA Kit
• Range: 31.25-2000 pg/mL
• Assay Time: 3.5h
• UniProt ID: P51532
• Sensitivity: 11.5 pg/mL
• Alternative names: ATP-dependent helicase SMARCA4;BRG1-associated fac
• Note: For research use only
• Application notes: standard: 2000 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human SMARCA4. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human SMARCA4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human SMARCA4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human SMARCA4 in the samples is then determined by comparing the OD of the samples to the standard curve
• Sample Types: serum, plasma, tissue homogenates, cell lysates
• Expiration Date: Please enquire.