Human GABA-A Receptor Associated Protein Like Protein 2 (GABARAPL2) ELISA Kit

SKU: orb778922

Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to GABA-A Receptor Associated Protein Like Protein 2(GABARAPL2).

Images & Validation

• Tested Applications: ELISA
• Application Notes:
  standard: 10 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human GABARAPL2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human GABARAPL2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human GABARAPL2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human GABARAPL2 in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

• Reactivity: Human
• Sample Types: Tissue homogenates, cell lysates and other biological fluids.
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 0.16-10 ng/mL
• Sensitivity: 0.063 ng/mL
• Concentration: 10 ng/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Disclaimer: For research use only

Alternative Names

ATG8 ELISA Kit, GATE16 ELISA Kit, GEF2 ELISA Kit, FLC3A ELISA Kit, General protein transport factor p16 ELISA Kit, Ganglioside expression factor 2 ELISA Kit, Golgi-associated ATPase enhancer of 16 kDa ELISA Kit, MAP1 light chain 3-related ELISA Kit