GAD67 Antibody / GAD1

SKU: orb606600

Description

This mAb recognizes a protein of 67kDa, which is identified as glutamic acid decarboxylase 1 (GDA1). There are two forms of glutamic acid decarboxylases (GADs) that are found in the brain: GAD65 (also known as GAD2) and GAD67 (also known as GAD1). GAD65 and GAD67 are members of the group II decarboxylase family of proteins and are responsible for catalyzing the rate-limiting step in the production of GABA (gamma-aminobutyric acid) from L-glutamic acid. Although both GAD s are found in the brain, GAD65 localizes to synaptic vesicle membranes in nerve terminals, while GAD67 is distributed throughout the cell. GAD67 is responsible for the basal levels of GABA synthesis. In the case of a heightened demand for GABA in neurotransmission, GAD65 will transiently activate to assist in GABA production. The loss of GAD65 is detrimental and can impair GABA neurotransmission, however the loss of GAD67 is lethal.

Research Area

Epigenetics, Neuroscience

Images & Validation

• Tested Applications: ELISA, FACS, WB
• Dilution range: ELISA (order BSA/sodium azide-free format for coating),Flow cytometry: 1-2ug/10^6 cells,Western blot: 0.5-1ug/ml
• Reactivity: Human
• Application Notes:
  Optimal dilution of the GAD67 antibody should be determined by the researcher.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgG2b, kappa
• Clone No.: GAD1/2563
• Immunogen: A portion of amino acids 72-135 from the human protein were used as the immunogen for this GAD67 antibody.
• Purification: Protein G affinity chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Disclaimer: For research use only

IHC testing of FFPE human brain with GAD67 antibody (clone GAD1/2563). Required HIER: boil tissue sections in 10mM citrate buffer, pH6, for 10-20 min.

Analysis of HuProt (TM) microarray containing more than 19, 000 full-length human proteins using GAD67 antibody (clone GAD1/2563). These results demonstrate the foremost specificity of the GAD1/2563 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt (TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt (TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

SDS-PAGE analysis of purified, BSA-free GAD67 antibody (clone GAD1/2563) as confirmation of integrity and purity.

Quick Database Links

UniProt

Q99259