Carmustine

SKU: orb1304551

Description

Carmustine (BCNU) is a non-specific alkylating agent used in cancer research. It is widely applied in vitro and in vivo for studying gliomas, lymphomas, and other malignancies, where it induces DNA cross-linking and apoptosis.

Research Area

Molecular Biology

Key Properties

• CAS Number: 154-93-8
• MW: 214.05
• Purity: 99.97% (May vary between batches)
• Formula: C₅H₉Cl₂N₃O₂
• SMILES: N(C(NCCCl)=O)(CCCl)N=O
• Target: DNA Alkylation,DNA Alkylator/Crosslinker
• Solubility: 10% DMSO+40% PEG300+5% Tween 80+45% Saline:2 mg/mL (9.34 mM);DMSO:62.5 mg/mL (291.99 mM)

Bioactivity

• Target IC50:
  DNA Alkylator:
• In Vivo:
  Carmustine (BCNU; 25 mg/kg, i.p.) increases the ratio of liver weight to body weight and levels of plasma conjugated bilirubin, while reducing biliary flow and oxidized glutathione levels (GSSG), along with a decrease in the ratio of reduced glutathione (GSH) to GSSG, compared to control rats.
• In Vitro:
  Carmustine is an antitumor chemotherapeutic agent. Carmustine (8, 80, and 800 μM) decreases N-acetyltransferase (NAT) activities for 2-aminofluorene (AF) and p-aminobenzoic acid (PABA) in rat glial tumor cytosol and intact cells. The carmustine decreases the formation of DNA-AF adduct when the DNA-AF adduct increases in rat glial tumor cell.
• Animal Research:
  Carmustine is formulated in corn oil.RatsIndividual rats are weighted prior to enter the study; their weights are recorded, and they are randomLy assigned to four groups. Group I (saline group); This group consists of 12 rats. These rats are injected with 2 mL/kg of saline intraperitoneally (IP) 48 h before the study, being included by the study 48 h later. Group II (corn oil group) consists of 15 rats. These rats are injected with 2 mL/kg of corn oil (vehicle) IP 48 h before the study. Group III (Carmustine group) consists of 16 rats. These rats are injected with 1 mL per day of saline IP, administered at the same hour of the day as a single-dose for 3 days. Twelve hours after the first dose of saline, corn oil 2 mL/kg + Carmustine 25 mg/kg IP are injected, and the rats are included in the study 48 h after the administration of corn oil + Carmustine. Group IV (trimetazidine group) consists of 12 rats. These rats are injected with 2.5 mg/kg per day of trimetazidine (TMZ) IP, administered at the same hour of the day as a single-dose for 3 days. 12 h after the first dose of TMZ, corn oil 2 mL/kg + Carmustine 25 mg/kg IP are injected, and the rats are included in the study 48 h after the administration of corn oil + Carmustine

Storage & Handling

• Storage: keep away from direct sunlight | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Carmubris, Carmustine, BCNU, BiCNU, bis-chloroethylnitrosourea, DNA Alkylator/Crosslinker, DNA Alkylator, DNA Alkylation, DNAAlkylation, DNAAlkylator, Crosslinker, Nitrumon, inhibit, Inhibitor, Gliadel

Compound Identifiers

PubChem CID

2578