Biotin NHS

SKU: orb1218127

Description

Biotin NHS is an amino reactive biotin reagent used in the preparation of biotinylated surfaces or polypeptides.

Key Properties

• CAS Number: 35013-72-0
• MW: 341.38
• Purity: >98% (HPLC)
• Formula: C₁₄H₁₉N₃O₅S
• SMILES: O=C(CCCC[C@@H]([C@H]1N2)SC[C@@H]1NC2=O)ON(C(CC1)=O)C1=O
• Target: others
• Solubility: DMSO:60 mg/mL (175.76 mM)

Bioactivity

• In Vitro:
  Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs). Calculations: For best results, use ≥ 12-fold molar excess of biotin for a 10 mg/mL protein solution or ≥ 20-fold molar excess of biotin for a 2 mg/mL protein solution.1. Calculate millimoles of biotin reagent to add to the reaction for a 20-fold molar excess: mL protein x (mg protein/mL protein) x (mmoL protein/mg protein) x (20 mmoL protein/mmoL protein) = mmoL Biotin. Calculate microliters of 10 mM biotin reagent solution (prepared in Step B.3) to add to the reaction: mmoL Biotin x (1000000 μL/L) x (L/ 10 mmoL) =μL Biotin Biotin Labeling Reaction: 1. Dissolve 1-10 mg protein in 0.5-2.0 mL PBS according to the calculation made in Calculations. 2. Before use, prepare a 10 mM solution of the biotin reagent in an organic solvent such as DMSO. 3. Add the appropriate volume (μL) of 10 mM biotin reagent solution to the protein solution. 4. Incubate reaction on ice for two hours or at room temperature for 30 minutes. To tests the labeled protein by ELISA or Western blot.
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Storage & Handling

• Storage: Storage temperature: -20°C. Stability: ≥ 2 years
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Biotin-NHS | Biotin N-hydroxysuccinimide ester | NHS-Biotin

Quick Database Links

Gene Symbol

others