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Biotin-dC-puromycin
SKU: orb533259
Description
Customer Validated Data
− The data below is submitted by researchers worldwide. We provide this transparency to help you decide if this product works for your specific application or species, even if we haven't tested it ourselves.
Success by Application
Reactivity Distribution
Latest Experiments
5 ResultsDilution
Sample
Summary
In vitro translation
E. coli
10 μM
In vitro translation reaction mixture containing ribosome–nascent chain complexes (RNCs)
Biotin-dC-puromycin was applied as a nascent...
Nascent Protein Labeling
Mouse
10 μM
Isolated ribosomes, Ribosome–nascent chain complexes from cultured cells
Biotin-dC-puromycin was used for nascent protein...
Nascent Protein Labeling
Human
1:1000
Whole-cell lysates
Biotin-dC-puromycin was applied for puromycin-based nascent...
Translatome Analysis
E. coli
10 µm
In vitro translation mixtures; isolated polysomes/ribosomes
Biotin-dC-puromycin is used as a nascent...
Interactome Analysis
Human
-
Human cells (HEK293T)
Biotin-dC-puromycin is used as a nascent...
Images & Validation
−Item 1 of 1
| Application Notes |
|---|
Key Properties
−| CAS Number | 436083-86-2 |
|---|---|
| MW | Theoretical MW: 1166.14 g/mol (free acid); Detected MW: 1165.42 g/mol (free acid) |
| Purity | ≥ 95% (HPLC) |
| Formula | C47H69N13O16P2S |
| SMILES | [C@@H]1(C[C@@H](O[C@@H]1COP(=O)(OCCCCCCNC(=O)CCCC[C@H]1[C@@H]2[C@H](CS1)NC(=O)N2)O)n1c(=O)nc(cc1)N)OP(=O)(O)OC[C@@H]1[C@H]([C@H]([C@@H](O1)n1c2ncnc(c2nc1)N(C)C)O)NC(=O)[C@H](Cc1ccc(cc1)OC)N |
Storage & Handling
−| Storage | Store at -20 °C. Short term exposure (up to 1 week cumulative) to ambient temperature possible. |
|---|---|
| Expiration Date | 12 months from date of receipt. |
| Hazard Information | Non-Toxic |
| Disclaimer | For research use only |
Similar Products
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Biotin-dC-puromycin [orb1733752]
≥ 95% (HPLC)
436083-86-2
Theoretical MW: 1166.14 g/mol (free acid); Detected MW: 1165.42 g/mol (free acid)
C47H69N13O16P2S
100 μl
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Structural formula of Biotin-dC-puromycin.
Compound Identifiers
PubChem CID
Key Properties
− No computed properties available.
Protocol Information
Biotin-dC-puromycin (orb533259)
- 5.0
Based on 1 review
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- 5 stars
The chemical easily labeled nascent peptides even at lower concentrations. I have repeated the experiments three times, and the results are consistent.
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