Anti-Phosphotyrosine Antibody

• Catalog Number: orb323189
• Category: Antibodies
• Description: Mouse monoclonal antibody to Phosphotyrosine
• Species/Host: Mouse
• Clonality: Monoclonal
• Tested applications: IH, WB
• Reactivity: All
• Immunogen: Purified protein corresponding to Phosphotyrosine.
• Antibody Type: Primary Antibody
• Dilution range: WB: 1:1000-1:2000, IHC-P: 1:50-1:200
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Source: Mouse
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of Phosphotyrosine expression in Jurkat (A), 293T (B) whole cell lysates. (Predicted band size: \; Observed band size: \)

Immunohistochemical analysis of Phosphotyrosine staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.