CD16 Antibody

SKU: orb44101

Description

Mouse Monoclonal to CD16.

Images & Validation

• Tested Applications: FA, FC, IP, WB
• Reactivity: Human
• Application Notes:
  Functional application: The antibody MEM-154 blocks binding of human IgG to FcγRIII. Flow cytometry: Recommended dilution: 5-10 μg/ml; positive control: PBL (peripheral blood lymphocytes). The antibody MEM-154 does not react with CD16a present on NK cells in many subjects. Western blotting: Non-reducing conditions.

Key Properties

• Antibody Type: Primary Antibody
• Clonality: Monoclonal
• Isotype: Mouse IgG1
• Clone No.: MEM-154
• Immunogen: Human granulocytes
• Target: CD16
• Purification: Purified by protein-A affinity chromatography.

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Phosphate buffered saline (PBS), pH 7.4
• Concentration: 1 mg/ml
• Disclaimer: For research use only

Alternative Names

FcgammaRIII, IGFR3, FCRIII

Separation of human neutrophil granulocytes (red-filled) from CD16 negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD16 (MEM-154) purified antibody (azide free, concentration in sample 2 μg/ml) GAM APC.

Flow cytometry surface staining pattern of human peripheral blood stained using anti-human CD16 (MEM-154) purified antibody (azide free, concentration in sample 2 μg/ml) GAM APC.

Quick Database Links

Gene Symbol

CD16

NCBI Gene ID

35