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Item 1 of 2
Anti-Hu CD16 Purified Azide Free
Catalog Number: orb44101
| Catalog Number | orb44101 |
|---|---|
| Category | Antibodies |
| Description | Mouse Monoclonal to CD16. |
| Clonality | Monoclonal |
| Clone Number | MEM-154 |
| Tested applications | FA, FC, IP, WB |
| Reactivity | Human |
| Isotype | Mouse IgG1 |
| Immunogen | Human granulocytes |
| Antibody Type | Primary Antibody |
| Concentration | 1 mg/ml |
| Purity | Purified by protein-A affinity chromatography. |
| Conjugation | Unconjugated |
| Target | CD16 |
| RRID | AB_10996078 |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Buffer/Preservatives | Phosphate buffered saline (PBS), pH 7.4 |
| Alternative names | Anti-CD16 antibody, anti-FACSgammaRIII antibody Read more... |
| Note | For research use only |
| Application notes | Functional application: The antibody MEM-154 blocks binding of human IgG to FcγRIII. Flow cytometry: Recommended dilution: 5-10 μg/ml; positive control: PBL (peripheral blood lymphocytes). The antibody MEM-154 does not react with CD16a present on NK cells in many subjects. Western blotting: Non-reducing conditions. |
| Expiration Date | 12 months from date of receipt. |
Separation of human neutrophil granulocytes (red-filled) from CD16 negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD16 (MEM-154) purified antibody (azide free, concentration in sample 2 μg/ml) GAM APC.
Flow cytometry surface staining pattern of human peripheral blood stained using anti-human CD16 (MEM-154) purified antibody (azide free, concentration in sample 2 μg/ml) GAM APC.
