Zebrafish 5-Hydroxytryptamine (5-HT) ELISA Kit

• Catalog Number: orb1146916
• Category: Assays and Kits
• Description: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Zebrafish 5-HT protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Zebrafish 5-HT. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Zebrafish 5-HT in the samples is then determined by comparing the OD of the samples to the standard curve.
• Reactivity: Zebrafish
• Concentration: 900 ng/mL
• Application notes: standard: 900 ng/mL. Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Zebrafish 5-HT protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Zebrafish 5-HT. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Zebrafish 5-HT in the samples is then determined by comparing the OD of the samples to the standard curve
• Assay Type: Competitive
• Assay Time: 2.5h
• Range: 14.07-900 ng/mL
• Sensitivity: 3.85 ng/mL
• Sample Types: serum, plasma, tissue homogenates
• Alternative names: 5HT; Serotonin; N-Methyl-Gamma
• Research Area: Gastroenterology & Hepatology, Infectious Disease
• Note: For research use only
• Expiration Date: Please enquire.