XIAP (BIRC4) Antibody (N-term)

• Catalog Number: orb1930240
• Category: Antibodies
• Description: Purified Rabbit Polyclonal Antibody (Pab)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Clone Number: RB01793
• Tested applications: IHC-P, WB
• Reactivity: Human, Mouse, Rat
• Isotype: Rabbit IgG
• Antibody Type: Primary Antibody
• Dilution range: WB: 1:2000, IHC-P: 1:50~100
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Conjugation: Unconjugated
• MW: 56685 Da
• Target: This XIAP (BIRC4) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human XIAP (BIRC4).
• UniProt ID: P98170
• NCBI: NP_001191330.1, NP_001158.2
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: E3 ubiquitin-protein ligase XIAP, 632-, Baculovira
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Formalin-fixed and paraffin-embedded human colon carcinoma tissue reacted with BIRC4 antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

All lanes: Anti-hBIRC4-C12 at 1:2000 dilution. Lane 1: MCF-7 whole cell lysate. Lane 2: Ramos whole cell lysate. Lane 3: C2C12 whole cell lysate. Lane 4: PC-12 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 57 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.