WT1 Antibody / Wilms Tumor 1

• Catalog Number: orb750181
• Category: Antibodies
• Description: Wilms tumor is associated with mutations of WT1, a zinc-finger transcription factor that is essential for the development of the metanephric kidney and the urogenital system. The gene is normally expressed in fetal kidney and mesothelium, and its expression has been suggested as a marker for Wilms tumor and mesothelioma. WT1 protein has been identified in proliferative mesothelial cells, malignant mesothelioma, ovarian carcinoma, gonadoblastoma, nephroblastoma, and desmoplastic small round cell tumor. Lung adenocarcinomas rarely stain positive with this antibody. WT1 protein expression in mesothelial cells has become a reliable marker for the diagnosis of mesotheliomas.
• Clonality: Recombinant
• Species/Host: Rabbit
• Isotype: Rabbit IgG, kappa
• Conjugation: Unconjugated
• Reactivity: Human, Rat
• Immunogen: A recombinant protein was used as the immunogen for the recombinant WT1 antibody.
• UniProt ID: P19544
• Tested applications: IHC-P
• Dilution range: Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
• Application notes: Optimal dilution of the recombinant WT1 antibody should be determined by the researcher.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Antibody Type: Primary Antibody
• Clone Number: WT1/1434R
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Hazard Information: This recombinant WT1 antibody is available for research use only.
• Note: For research use only

IHC testing of FFPE human mesothelioma tissue with recombinant WT1 antibody (clone WT1/1434R). HIER: steam sections in pH9 10 mM Tris with 1 mM EDTA for 10-20 min.

IHC testing of FFPE rat testis tissue with recombinant WT1 antibody (clone WT1/1434R). HIER: steam sections in pH9 10 mM Tris with 1 mM EDTA for 10-20 min.

SDS-PAGE analysis of purified, BSA-free recombinant WT1 antibody (clone WT1/1434R) as confirmation of integrity and purity.