VPS35 Antibody: HRP

• Catalog Number: orb612803
• Category: Antibodies
• Description: Mouse monoclonal antibody against VPS35 conjugated to HRP
• Target: VPS35
• Clonality: Monoclonal
• Species/Host: Mouse
• Isotype: IgG1
• Conjugation: HRP
• Reactivity: Human, Mouse, Rat
• Concentration: 1 mg/ml
• Buffer/Preservatives: 73.64mM Carbonate, 54.55mM Ethanolamine, 45.45mM Cyanoborohydride, 18.18mM Sodium Hydroxide and 0.23mM Citrate in dH2O
• Purification: Protein G Purified
• Immunogen: Full length recombinant human VSP35
• UniProt ID: Q96QK1
• MW: 92 kDa
• Tested applications: ELISA, IHC, WB
• Dilution range: WB (1:1000); ICC/IF (1:200); IP (1:200); IHC (1:100)
• Application notes: A 1:1000 dilution of SMC-604 was sufficient for detection of VPS35 in 10 µg of SH-SY5Y by ECL immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary antibody.
• Clone Number: 8A3
• Storage: Conjugated antibodies should be stored according to the product label
• Alternative names: Vacuolar protein sorting-associated protein 35, ME
• Research Area: Cell Biology, Neuroscience, Protein Biochemistry,
• Note: For research use only
• Entrez: 55737
• NCBI: NP_060676.2
• Expiration Date: 12 months from date of receipt.

Immunohistochemistry analysis using Mouse Anti-VPS35 Monoclonal Antibody, Clone 8A3. Tissue: Kidney. Species: Mouse. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody at 1:100 for Overnight at 4°C, then 30 min at 37°C. Secondary Antibody: Goat Anti-Mouse IgG (H+L): FITC for 45 min at 37°C. Counterstain: DAPI for 3 min at RT. Magnification: 20X.

Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-VPS35 Monoclonal Antibody, Clone 8A3. Tissue: A549 cells. Species: Human. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody at 1:5 (tissue culture supernatant). Secondary Antibody: Donkey anti-mouse: Alexa Fluor 594 at 1:4000 in 0.2% BSA PBS. Counterstain: DAPI. Localization: Vesicles. A) VPS35 KO A549 cells B) WT A549 cells.

Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-VPS35 Monoclonal Antibody, Clone 8A3. Tissue: A549 WT, VPS35 KO cells. Species: Human. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody. Secondary Antibody: Donkey Anti-Mouse AlexaFluor 594. Clone can detect VPS35 at 1/2000 concentration.

Immunoprecipitation analysis using Mouse Anti-VPS35 Monoclonal Antibody, Clone 8A3. Tissue: A549 cells. Species: Human. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody.

Western Blot analysis of Human, Mouse A549, MEF showing detection of VPS35 protein using Mouse Anti-VPS35 Monoclonal Antibody, Clone 8A3. Lane 1: Molecular Weight Ladder. Lane 2: VPS35 KO A549 cells. Lane 3: mouse embryonic fibroblast cells. Load: 8 μg each A549 and MEF. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody at 1:5 (tissue culture supernatant). Secondary Antibody: Donkey anti-mouse IRDye 800CW at 1:25000 in TBS-T.

Western Blot analysis of Human SH-SY5Y showing detection of VPS35 protein using Mouse Anti-VPS35 Monoclonal Antibody, Clone 8A3. Lane 1: Molecular Weight Ladder. Lane 2: SH-SY5Y (10 ug). Load: 10 μg. Block: 5% Skim Milk powder in TBST. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody at 1:1000 for 2 hours at RT with shaking. Secondary Antibody: Goat anti-mouse IgG:HRP at 1:4000 for 1 hour at RT with shaking. Color Development: Chemiluminescent for HRP (Moss) for 5 min in RT.

Immunoprecipitation analysis using Mouse Anti-VPS35 Monoclonal Antibody, Clone 8A3. Tissue: A549 cells. Species: Human. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody. Three amounts of (3, 1 and 0.3 ug) were non-covalently coupled to 10uL of A/G sepharose beads for 1 hour at 4°C and next incubated with 250ug of A549 lysate for 2 hours at 4°C.

Immunoprecipitation analysis using Mouse Anti-VPS35 Monoclonal Antibody, Clone 8A3. Tissue: embryonic fibroblast. Species: Mouse. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody. Three amounts of (3, 1 and 0.3 ug) were non-covalently coupled to 10uL of A/G sepharose beads for 1 hour at 4°C and next incubated with 250ug of MEF lysate for 2 hours at 4°C.

Immunoprecipitation analysis using Mouse Anti-VPS35 Monoclonal Antibody, Clone 8A3. Tissue: A549 cells. Species: Human. Primary Antibody: Mouse Anti-VPS35 Monoclonal Antibody. 500 μL cell culture supernatants were incubated with 10 μL of Protein A/G resin beads for 1 hour at 4°C.