VDAC2 Antibody (N-term)

• Catalog Number: orb1926260
• Category: Antibodies
• Description: Purified Rabbit Polyclonal Antibody (Pab)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Clone Number: RB49629
• Tested applications: IHC-P, WB
• Reactivity: Human, Mouse, Rat
• Isotype: Rabbit IgG
• Antibody Type: Primary Antibody
• Dilution range: WB: 1:4000, WB: 1:2000, IHC-P: 1:100, IHC-P: 1:100
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Conjugation: Unconjugated
• MW: 31567 Da
• Target: This VDAC2 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 51-85 amino acids from the N-terminal region of human VDAC2.
• UniProt ID: P45880
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Voltage-dependent anion-selective channel protein
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Immunohistochemical analysis on paraffin-embedded Human brain tissue. Tissue was fixed with formaldehyde at room temperature. Heat induced epitope retrieval was performed by EDTA buffer (pH9.0). Samples were incubated with primary antibody (1:100) for 1 hour at room temperature. Undiluted CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.

Immunohistochemical analysis on paraffin-embedded Human kidney tissue. Tissue was fixed with formaldehyde at room temperature. Heat induced epitope retrieval was performed by EDTA buffer (pH9.0). Samples were incubated with primary antibody (1:100) for 1 hour at room temperature. Undiluted CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.

All lanes: Anti-VDAC2 Antibody (N-term) at 1:2000 dilution. Lane 1: HCT116 whole cell lysate. Lane 2: NCI-H1299 whole cell lysate. Lane 3: PC-3 whole cell lysate. Lane 4: Mouse brain tissue lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 32 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

All lanes: Anti-VDAC2 Antibody (N-term) at 1:4000 dilution. Lane 1: NCI-H1299 whole cell lysate. Lane 2: HCT116 whole cell lysate. Lane 3: PC-3 whole cell lysate. Lane 4: Mouse heart tissue lysate. Lane 5: Rat heart tissue lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 32 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.