UVRAG Antibody: Biotin

• Catalog Number: orb377189
• Category: Antibodies
• Description: Rabbit polyclonal antibody against UVRAG conjugated to Biotin.
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, ICC, IF, IHC, WB
• Reactivity: Human, Mouse, Rat
• Immunogen: Synthetic peptide from the mid-protein of human UVRAG
• Concentration: 1 mg/ml
• Dilution range: WB (1:1000); ICC/IF (1:100)
• Conjugation: Biotin
• MW: 78.1kDa
• Target: UVRAG
• Entrez: 7405
• UniProt ID: Q9P2Y5
• NCBI: NP_003360.2
• Storage: Conjugated antibodies should be stored according to the product label
• Buffer/Preservatives: 136.36mM Ethanolamine, and 9.55mM Sodium Bicarbonate in 95.45% PBS
• Alternative names: DHTX antibody, p63 antibody, UV radiation resistan
• Note: For research use only
• Application notes: A 1:1000 dilution of SPC-606 was sufficient for detection of UVRAG on HeLa cell lysates using Goat anti-rabbit IgG:HRP as the secondary antibody.
• Expiration Date: 12 months from date of receipt.

Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-UVRAG Polyclonal Antibody. Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-UVRAG Polyclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Late Endosome, Lysosome, Early Endosome. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) UVRAG Antibody (D) Composite.

Western blot analysis of Human HeLa and HEK293T cell lysates showing detection of ~100kDa UVRAG protein using Rabbit Anti-UVRAG Polyclonal Antibody. Lane 1: MW Ladder. Lane 2: Human HeLa (20 μg). Lane 3: Human 293T (20 μg). Load: 20 μg. Block: 5% Skim Milk for 1 hour at RT. Primary Antibody: Rabbit Anti-UVRAG Polyclonal Antibody at 1:200 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:2000 for 1 hour at RT. Color Development: ECL solution for 6 min at RT. Predicted/Observed Size: ~100kDa.

Western blot analysis of Rat Liver showing detection of ~100kDa UVRAG protein using Rabbit Anti-UVRAG Polyclonal Antibody. Lane 1: MW Ladder. Lane 2: Rat Liver (20 μg). Load: 20 μg. Block: 5% Skim Milk for 1 hour at RT. Primary Antibody: Rabbit Anti-UVRAG Polyclonal Antibody at 1:200 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:2000 for 1 hour at RT. Color Development: ECL solution for 6 min at RT. Predicted/Observed Size: ~100kDa.