TYRP1/gp75 Antibody

• Catalog Number: orb1538222
• Category: Antibodies
• Description: TYRP1/gp75 Antibody
• Target: TYRP1 / gp75
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse
• Concentration: 0.5 mg/ml
• Buffer/Preservatives: PBS, 0.09% sodium azide, 2% sucrose. PBS, 0.09% sodium azide, 2% sucrose
• Purification: Immunoaffinity purified
• Immunogen: Synthetic peptide located between aa396-445 of human TYRP1 (P17643, NP_000541). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Sheep, Goat, Panda, Dog, Cat, Bat, Rabbit, Horse, Guinea pig, Chicken, Platypus (100%); Elephant, Bovine, Pig, Opossum, Zebra finch, Lizard (92%); Xenopus (85%); Pufferfish, Zebrafish (83%).
• Tested applications: IHC, IHC-P, WB
• Dilution range: IHC, IHC-P (5 µg/ml), WB (1 µg/ml)
• Application notes: Further information: Immunohistochemistry: LS-B4011 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B4011 was determined to be 5 ug/ml. Western Blot: Suggested dilution at 1µg/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:312500.
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: TYRP1, B-PROTEIN, CAS2, CATB, Glycoprotein 75, Hst
• Note: For research use only