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TYROBP Rabbit Polyclonal Antibody (FITC)

Catalog Number: orb464992

DispatchUsually dispatched within 2-3 weeks
$ 450.00
Catalog Numberorb464992
CategoryAntibodies
DescriptionTYROBP Rabbit Polyclonal Antibody (FITC)
Species/HostRabbit
ClonalityPolyclonal
Tested applicationsFC
Predicted ReactivityBovine, Canine, Equine, Human, Porcine, Rat, Sheep
ReactivityMouse
IsotypeIgG
ImmunogenKLH conjugated synthetic peptide derived from human DAP12 (31-113/113aa)
Antibody TypePrimary Antibody
Concentration1mg/ml
Dilution rangeFlow-Cyt=1μg/Test
ConjugationFITC
TargetTYROBP
StorageShipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Buffer/Preservatives0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Alternative namesDAP 12; DAP12; DNAX activation protein 12; DNAX-ac
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NoteFor research use only
Expiration Date12 months from date of receipt.
TYROBP Rabbit Polyclonal Antibody (FITC)

Blank control (Black line): U937 (Black). Primary Antibody (green line): Rabbit Anti-DAP12 Conjugated FITC antibody (orb464992), dilution: 3 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG-FITC. Protocol, The cells were fixed with 4% PFA (10 min at room temperature). The cells were then incubated in 5% BSA to block non-specific protein-protein interactions followed by the antibody for 15 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. Acquisition of 20000 events was perf.

TYROBP Rabbit Polyclonal Antibody (FITC)

Blank control: HL-60. Primary Antibody (green line): Rabbit Anti-DAP12 antibody (orb464992), dilution: 2 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Protocol, The cells were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. Acquisition of 20000 events was performed.