TUBB3 (Neuronal Marker) Rabbit Polyclonal Antibody (BF594)

• Catalog Number: orb1587539
• Category: Antibodies
• Description: TUBB3 (Neuronal Marker) Rabbit Polyclonal Antibody (BF594)
• Target: TUBB3
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: BF594
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Canine, Rabbit
• Concentration: 1mg/ml
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Purification: Affinity purified by Protein A
• Immunogen: KLH conjugated synthetic peptide derived from human beta III Tubulin (401-450/450aa)
• Tested applications: FC, ICC, IF
• Dilution range: Flow-Cyt=1μg/Test, IF=1:200-800, ICC/IF=1:100-500
• Antibody Type: Primary Antibody
• Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
• Alternative names: Neuron specific beta III Tubulin; beta 4; MC1R; TB
• Research Area: Neurology, Neuroscience, Signal Transduction
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Paraformaldehyde-fixed, paraffin embedded (Mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Incubation: Anti-TUBB3 Antibody, conjugated 1:400, 1.5 hours at 37°C, DAPI (5 ug/ml, blue) was used to stain the cell nuclei.

Tissue/Cell: U251 cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-TUBB3 (Neuronal Marker) Polyclonal Antibody, conjugated (orb1587539) 1:200, 1.5 hours at 37°C, DAPI (5 ug/ml, blue) was used to stain the cell nuclei.