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Item 1 of 2
TUBB3 (Neuronal Marker) Rabbit Polyclonal Antibody (BF594)
Catalog Number: orb1587539
| Catalog Number | orb1587539 |
|---|---|
| Category | Antibodies |
| Description | TUBB3 (Neuronal Marker) Rabbit Polyclonal Antibody (BF594) |
| Species/Host | Rabbit |
| Clonality | Polyclonal |
| Tested applications | FC, ICC, IF |
| Predicted Reactivity | Canine, Rabbit |
| Reactivity | Human, Mouse, Rat |
| Isotype | IgG |
| Immunogen | KLH conjugated synthetic peptide derived from human beta III Tubulin (401-450/450aa) |
| Antibody Type | Primary Antibody |
| Concentration | 1mg/ml |
| Dilution range | Flow-Cyt=1μg/Test, IF=1:200-800, ICC/IF=1:100 |
| Conjugation | BF594 |
| Target | TUBB3 |
| Storage | Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Buffer/Preservatives | 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol. |
| Alternative names | Neuron specific beta III Tubulin; beta 4; MC1R; TB Read more... |
| Note | For research use only |
| Expiration Date | 12 months from date of receipt. |
Paraformaldehyde-fixed, paraffin embedded (Mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Incubation: Anti-TUBB3 Antibody, conjugated 1:400, 1.5 hours at 37°C, DAPI (5 ug/ml, blue) was used to stain the cell nuclei.
Tissue/Cell: U251 cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-TUBB3 (Neuronal Marker) Polyclonal Antibody, conjugated (orb1587539) 1:200, 1.5 hours at 37°C, DAPI (5 ug/ml, blue) was used to stain the cell nuclei.
