Trypsin Inhibitor Antibody

• Catalog Number: orb750596
• Category: Antibodies
• Description: KTI3 antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, WB
• Reactivity: Plant
• Isotype: Antiserum
• Immunogen: Trypsin Inhibitor [Soy Bean]
• Antibody Type: Primary Antibody
• Concentration: 85 mg/mL
• Dilution range: ELISA: 1:3,000 - 1:15,000, WB: 1:500 - 1:2,000
• Form/Appearance: Lyophilized
• Purity: This product was prepared from monospecific antiserum by a delipidation and defibrination. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-rabbit serum, purified and partially purified Trypsin Inhibitor [Soy Bean]. Cross reactivity against Trypsin Inhibitor from other tissues and species may occur but have not been specifically determined.
• Conjugation: Unconjugated
• UniProt ID: P01070
• NCBI: P01070.2
• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: rabbit anti-Trypsin Inhibitor Antibody, 25 kDa try
• Note: For research use only
• Application notes: Anti-Trypsin Inhibitor has been tested by western blot and is suitable to be assayed against 1.0 ug of Trypsin Inhibitor [Lima Bean] in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) code #611-1302 and (ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:200 to 1:1,000 of the reconstitution concentration is suggested for this product.
• Expiration Date: 12 months from date of receipt.

Biorbyt Biotin conjugated Rabbit anti-trypsin inhibitor antibody and Rabbit anti streptavidin were used to detect target proteins Trypsin Inhibitor (left) and Streptavidin (right) under reducing (R) and non-reducing (NR) conditions. Reduced samples of purified target proteins contained 4% BME and were boiled for 5 minutes. Samples of ~1 ug of protein per lane were run by SDS-PAGE. Protein was transferred to nitrocellulose and probed with 1:1000 dilution of primary antibody (ON 4 C). Detection shown was using Dylight 649 conjugated Donkey anti rabbit (1:10K 1.5 hr RT in orb348637).

Biorbyt Rabbit anti Soybean Trypsin Inhibitor antibody was used to detect purified Soybean Trypsin Inhibitor. Samples of ~1 and 0.25 ug of purified Soybean Trypsin Inhibitor per lane were run by SDS-PAGE under reducing and non-reducing conditions and reduced samples of protein contained 4% BME and were boiled for 5 minutes. Protein was transferred to nitrocellulose and probed with Biorbyt Rabbit anti Soybean Trypsin Inhibitor antibody (1:5K, ON 4 C). Primary antibody was detected with Biorbyt Dylight 488 conjugated Streptavidin (1:5K) and Atto 425 conjugated goat anti rabbit (1:10K 1.5 hr RT in orb348637).