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Catalog Number | orb766514 |
---|---|
Category | Antibodies |
Description | Rabbit polyclonal antibody to TRAP100 |
Clonality | Polyclonal |
Tested applications | ELISA, IF, IHC-P, WB |
Reactivity | Human, Mouse, Rat |
Isotype | IgG |
Immunogen | The antiserum was produced against synthesized peptide derived from human MED24. AA range:801-850 |
Concentration | 1 mg/ml |
Dilution range | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications. |
Conjugation | Unconjugated |
MW | 110 |
Source | Rabbit |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Buffer/Preservatives | PBS with 0.02% sodium azide and 50% glycerol pH 7.4. |
Alternative names | Anti-MED24 antibody, anti-ARC100 antibody, anti-CR Read more... |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
Western Blot analysis of various cells using TRAP100 Polyclonal Antibody diluted at 1:1000. Secondary antibody was diluted at 1:20000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit.
Immunofluorescence analysis of HUVEC cells, using MED24 Antibody. The picture on the right is blocked with the synthesized peptide.
Western blot analysis of lysates from 293 cells, using MED24 Antibody. The lane on the right is blocked with the synthesized peptide.
Immunohistochemical analysis of paraffin-embedded human Colon cancer. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).