TOP2A Recombinant Rabbit Monoclonal Antibody

• Catalog Number: orb2563016
• Category: Antibodies
• Description: TOP2A Recombinant Rabbit Monoclonal Antibody
• Target: TOP2A
• Clonality: Recombinant
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Mouse, Rat
• Form/Appearance: Liquid
• Concentration: 1mg/ml
• Buffer/Preservatives: 0.01M TBS(pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Purification: Affinity purified by Protein A
• Immunogen: A synthesized peptide derived from human Topoisomerase 2 alpha (1500-1531/1531aa)
• UniProt ID: P11388
• MW: 174 kDa
• Tested applications: IF, IHC-Fr, IHC-P, WB
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Antibody Type: Primary Antibody
• Clone Number: 11D3
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: DNA topoisomeraseⅡalpha; TOPO II; DNA topoisomeras
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

25 ug total protein per lane of various lysates probed with TOP2A monoclonal antibody, unconjugated (orb2563016) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

Paraformaldehyde-fixed, paraffin embedded Human Testicles, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with TOP2A Monoclonal Antibody, Unconjugated (orb2563016) at 1:400 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.

Paraformaldehyde-fixed, paraffin embedded Mouse Lymph, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with TOP2A Monoclonal Antibody, Unconjugated (orb2563016) at 1:400 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.

Paraformaldehyde-fixed, paraffin embedded Mouse Testicles, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with TOP2A Monoclonal Antibody, Unconjugated (orb2563016) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.

Paraformaldehyde-fixed, paraffin embedded Rat Lymph, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with TOP2A Monoclonal Antibody, Unconjugated (orb2563016) at 1:400 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.

Paraformaldehyde-fixed, paraffin embedded Rat Testicles, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with TOP2A Monoclonal Antibody, Unconjugated (orb2563016) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.