Thymidylate Synthase Antibody

• Catalog Number: orb750616
• Category: Antibodies
• Description: TYMS antibody
• Species/Host: Sheep
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Reactivity: Human
• Isotype: Antiserum
• Immunogen: This whole sheep serum was prepared by repeated immunizations with recombinant human Thymidylate Synthase (36 kDa) produced in E.coli.
• Antibody Type: Primary Antibody
• Concentration: 85 mg/mL
• Dilution range: ELISA: 1:10,000 - 1:50,000, IHC: 1:100, WB: 1:500- 1:2,000
• Form/Appearance: Lyophilized
• Purity: This antiserum is directed against human Thymidylate Synthase and is useful in determining its presence in various assays. Because inhibition of Thymidylate Synthase prevents DNA synthesis and cell proliferation, the enzyme is an important target for cancer chemotherapeutic drugs, specifically the fluoropyrimidine group of antineoplastic drugs used to treat solid tumors. In general, this antibody can detect antigen in a variety of human cells and tissues, as well as bacteria, African green monkey, rat and mouse. Somewhat lower dilutions may be required in some non-human cell lines. Anti-Thymidylate Synthase can detect Thymidylate Synthase by immunochemistry in proliferating cell cultures and tissues but does not stain nonproliferating cells. Normal colon mucosa shows weak staining; however, some colorectal cancer specimens show very strong staining.
• Conjugation: Unconjugated
• UniProt ID: P04818
• NCBI: P04818.3
• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: sheep anti-Thymidylate Synthase Antibody, HsT422 a
• Note: For research use only
• Application notes: This antiserum against Human Thymidylate Synthase (hTS) has been tested in Western Blot and IHC. This antibody is suitable for use in ELISA, immunoprecipitation, and immunofluorescence microscopy. The antibody recognizes the expected additional band corresponding to the ternary complex of hTS-dFUMP-reduced folate in HeLa cells treated with the TS inhibitor 5-FUdR. This event occurs in most human breast, colorectal, gastric, head and neck carcinomas. The antibody recognizes the 36,000 MW hTS. Reactivity in other immunoassays is unknown.
• Expiration Date: 12 months from date of receipt.

Anti-Thymidylate Synthase is shown to detect thymidylate synthase present in a HeLa cell lysate (p/n orb348668). Each lane is estimated to contain 4 µg of protein. Lanes 1, 2 and 3 represent 1:2000, 1:5000 and 1:10000 fold dilutions of the antibody. Detection was made using HRP Rabbit-a-Sheep IgG (p/n orb347924) diluted 1:1000 and color development using TMB (orb348656) substrate for approximately 4'.

Immunohistochemistry of Thymidylate Synthase antibody. Tissue: human Kidney. Fixation: formalin fixed paraffin embedded. Antigen retrieval: user optimized. Primary antibody: orb750616 Thymidylate antibody at 1:100. Secondary antibody: Peroxidase goat anti-sheep at 1:10000 for 45 min at RT.