Thymidylate Synthase Antibody

• Catalog Number: orb345028
• Category: Antibodies
• Description: Thymidylate Synthase antibody
• Species/Host: Sheep
• Clonality: Polyclonal
• Tested applications: ELISA, WB
• Reactivity: Human
• Isotype: IgG
• Immunogen: Anti-THYMIDYLATE SYNTHASE was purified from rabbit serum after repeated immunizations with recombinant human Thymidylate Synthase (36 kDa) produced in E.coli.
• Antibody Type: Primary Antibody
• Concentration: 1 mg/ml
• Dilution range: ELISA: 1:10,000 - 1:50,000, WB: 1:500 - 1:2,000
• Form/Appearance: Lyophilized
• Purity: Anti-THYMIDYLATE SYNTHASE IgG fraction is directed against human Thymidylate Synthase and is useful in determining its presence in various assays. Because inhibition of Thymidylate Synthase prevents DNA synthesis and cell proliferation, the enzyme is an important target for cancer chemotherapeutic drugs, specifically the fluoropyrimidine group of antineoplastic drugs used to treat solid tumors. In general, this antibody can detect antigen in a variety of human cells and tissues, as well as bacteria, African green monkey, rat and mouse. Somewhat lower dilutions may be required in some non-human cell lines. Anti-Thymidylate Synthase can detect Thymidylate Synthase by immunochemistry in proliferating cell cultures and tissues but does not stain nonproliferating cells. Normal colon mucosa shows weak staining; however, some colorectal cancer specimens show very strong staining.
• Conjugation: Unconjugated
• UniProt ID: P04818
• NCBI: P04818.3
• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: sheep anti-Thymidylate Synthase Antibody, d TMP sy
• Note: For research use only
• Application notes: THYMIDYLATE SYNTHASE antibody has been tested by western blot. This antibody is suitable for use in ELISA, immunoprecipitation, immunofluorescence microscopy, and immunohistochemistry. The antibody recognizes the expected additional band corresponding to the ternary complex of hTS-dFUMP-reduced folate in HeLa cells treated with the TS inhibitor 5-FUdR. This event occurs in most human breast, colorectal, gastric, head and neck carcinomas. The antibody recognizes the 36,000 MW hTS. Reactivity in other immunoassays is unknown.
• Expiration Date: 12 months from date of receipt.

Anti-TS is shown to detect thymidylate synthase present in a HeLa cell extract. Each lane is estimated to contain 4 ug of protein. Lanes 1, 2 and 3 represent 1:2000, 1:5000 and 1:10000 fold dilutions of the antibody. Detection was made using HRP Rabbit-a-Sheep IgG (orb347924) diluted 1:1000 and color development using TMB (orb348656) substrate for approximately 4'.

Western Blot results of Sheep anti-Thymidylate Synthase Antibody. Lane 1: Opal Prestained Molecular Weight Ladder. Lane 2: HeLa WCL (p/n orb348668). Lane 3: MOLT-4 WCL (p/n orb348696). Load: 10 µg/lane. Primary Antibody: Sheep anti-Thymidylate Synthase at 1 µg/ml overnight at 4°C. Secondary Antibody: Donkey anti-Sheep HRP (p/n orb347953) at 1:40000 for 30 min at RT. Blocking: BlockOut (p/n orb348644) for 30 min at RT. Predicted MW: ~36kDa. Observed MW: ~35kDa.