Thymidine Phosphorylase Antibody / PD-ECGF

• Catalog Number: orb699607
• Category: Antibodies
• Description: Recognizes a protein (amino acid 482) of 55kDa (in vivo 110kDa homodimer), identified as platelet-derived endothelial growth factor (PD-ECGF), same as thymidine phosphorylase (TP) or gliostatin. In the presence of inorganic orthophosphate, it catalyzes the reversible phospholytic cleavage of thymidine and deoxyuridine to their corresponding bases and 2-deoxyribose-1-phosphate. It is both chemotactic and mitogenic for endothelial cells and a non-heparin binding angiogenic factor present in platelets. Its enzymatic activity is crucial for angiogenic activity (metabolite is angiogenic). Higher levels of serum TP/PD-ECGF are observed in cancer patients. It is also involved in transformation of fluoropyrimidines, cytotoxic agents used in the treatment of a variety of malignancies, into active cytotoxic metabolites (e.g. 5-deoxy-5-fluorouridine to 5-FU). High intra-cellular levels of TP/PD-ECGF are associated with increased chemosensitivity to such antimetabolites.
• Clonality: Recombinant
• Species/Host: Mouse
• Isotype: Mouse IgG1, kappa
• Conjugation: Unconjugated
• Reactivity: Human
• Immunogen: Recombinant full-length human protein was used as the immunogen for the recombinant PD-ECGF antibody.
• UniProt ID: P19971
• Tested applications: IHC-P, WB
• Dilution range: Immunohistochemistry (FFPE): 1-2ug/ml for 30 minutes at RT,Western blot: 1-2ug/ml
• Application notes: Optimal dilution of the recombinant PD-ECGF antibody should be determined by the researcher.
• Antibody Type: Primary Antibody
• Clone Number: rTYMP/3444
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Research Area: Cancer, Developmental Biology, Epigenetics, Neuros
• Note: For research use only

Western blot testing of human spleen lysate with recombinant PD-ECGF antibody (clone rTYMP/3444). Predicted molecular weight ~55 kDa.

IHC staining of FFPE human placenta with recombinant PD-ECGF antibody (clone rTYMP/3444). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

SDS-PAGE analysis of purified, BSA-free recombinant PD-ECGF antibody (clone rTYMP/3444) as confirmation of integrity and purity.