Thermolabile UNG (Uracil N-Glycosylase) - 10 u/µl

• Catalog Number: orb1821423
• Category: Tools
• Description: Thermolabile UNG (UDG) is used in real-time PCR to prevent carry-over contamination of dU-containing DNA from previous reactions. The enzyme is fully active at temperatures up to 55°C and inactivated above 65°C. Thermolabile Uracyl N-Glycosylase (UNG, UDG) catalyzes the release of uracil from single and double stranded uracyl-containing DNA. The resulting abasic sites are susceptible to hydrolytic cleavage at elevated temperatures. An amount of 1 unit UNG completely digests 104to 106copies of U-containing DNA fragments in 2 min at 50°C.
• Form/Appearance: liquid
• Concentration: 10 unit/µl
• Storage: store at -20 °C. avoid freeze/thaw cycles
• Note: For research use only