TGM2 monoclonal antibody (M10), clone 2F4

• Catalog Number: orb2292055
• Category: Antibodies
• Description: Mouse monoclonal antibody raised against a partial recombinant TGM2.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 2F4
• Tested applications: ELISA, IF, IHC-P, WB
• Reactivity: Human
• Isotype: IgG2a Kappa
• Immunogen: TGM2 (AAH03551, 1 a.a. ~ 120 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
• Conjugation: Unconjugated
• Protein Sequence: MAEELVLERCDLELETNGRDHHTADLCREKLVVRRGQPFWLTLHFEGRNYEASVDSLTFSVVTGPAPSQEAGTKARFPLRDAVEEGDWTATVVDQQDCTLSLQLTTPANAPIGLYRLSLE
• NCBI: AAH03551
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: In 1x PBS, pH 7.4
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Detection limit for recombinant GST tagged TGM2 is approximately 0.03 ng/ml as a capture antibody.

Immunofluorescence of monoclonal antibody to TGM2 on HeLa cell. [antibody concentration 10 ug/ml]

Immunoperoxidase of monoclonal antibody to TGM2 on formalin-fixed paraffin-embedded human placenta. [antibody concentration 3 ug/ml]

Western Blot analysis of TGM2 expression in transfected 293T cell line by TGM2 monoclonal antibody (M10), clone 2F4. Lane 1: TGM2 transfected lysate (61.7 KDa). Lane 2: Non-transfected lysate.

Western Blot detection against Immunogen (38.83 KDa).