STIP1 Recombinant Rabbit Monoclonal Antibody

• Catalog Number: orb1592789
• Category: Antibodies
• Description: STIP1 Recombinant Rabbit Monoclonal Antibody
• Target: STIP1
• Clonality: Recombinant
• Species/Host: Rabbit
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Mouse, Rat
• Form/Appearance: Liquid
• Concentration: 1mg/ml
• Buffer/Preservatives: PBS, Glycerol, rAlbumin.
• Immunogen: KLH conjugated synthetic peptide derived from human STIP1
• UniProt ID: P11831
• MW: 63 kDa
• Tested applications: FC, ICC, IF, IHC-Fr, IHC-P, WB
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:400-800, ICC/IF=1:100-500, IF=1:100-500, Flow-Cyt=1:50-100
• Antibody Type: Recombinant Antibody
• Clone Number: B5C7
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Hop; Hsc70/Hsp90 organizing protein; Hsc70/Hsp90-o
• Note: For research use only

Cell line: HeLa, Fixation: 4% Paraformaldehyde, Permeabilization: 0.1% TritonX-100, Primary Ab Dilution: 1:500, Primary Ab incubation condition: 4°C, overnight, Secondary Ab: Goat Anti-Rabbit IgG, Nuclear counter stain: DAPI (Blue), Comment: Color green is the positive signal for orb1592789.

Cell line: HeLa, Fixation: 4% Paraformaldehyde, Permeabilization: 90% Methanol, Primary Ab Dilution: 1:100, Secondary Ab: Goat Anti-Rabbit IgG, Unlabelled control: The cell without incubation, with primary antibody and secondary antibody (Black line). Isotype control: Rabbit monoclonal IgG (Blue line).

Cell line: Neuro-2a, Fixation: 4% Paraformaldehyde, Permeabilization: 0.1% TritonX-100, Primary Ab Dilution: 1:500, Primary Ab incubation condition: 4°C, overnight, Secondary Ab: Goat Anti-Rabbit IgG, Nuclear counter stain: DAPI (Blue), Comment: Color green is the positive signal for orb1592789.

Sample: Lane 1: Mouse Testis tissue lysates, Lane 2: Mouse Cerebrum tissue lysates, Lane 3: Rat Testis tissue lysates, Lane 4: Human A549 cell lysates, Lane 5: Human A431 cell lysates, Lane 6: Human 293T cell lysates, Lane 7: Human HepG2 cell lysates, Lane 8: Human Jurkat cell lysates, Primary: Anti-STIP1 (orb1592789) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 63 kDa, Observed band size: 63 kDa.

Tissue: Human testis, Section type: Formalin fixed & Paraffin embedded section, Retrieval method: High temperature and high pressure, Retrieval buffer: Tris/EDTA buffer, pH9.0, Primary Ab Dilution: 1:1000, Primary Ab incubation condition: 1 hour at room temperature, Secondary Ab: SP Kit (Rabbit), Counter stain: Hematoxylin (Blue), Comment: Color brown is the positive signal for orb1592789.

Tissue: Rat testis, Section type: Formalin fixed & Paraffin embedded section, Retrieval method: High temperature and high pressure, Retrieval buffer: Tris/EDTA buffer, pH9.0, Primary Ab Dilution: 1:1000, Primary Ab incubation condition: 1 hour at room temperature, Secondary Ab: SP Kit (Rabbit), Counter stain: Hematoxylin (Blue), Comment: Color brown is the positive signal for orb1592789.