SDF-1B Antibody

• Catalog Number: orb1272312
• Category: Antibodies
• Description: SDF-1B Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, WB
• Reactivity: Human
• Immunogen: Produced from sera of goats pre-immunized with highly pure (>98%) recombinant hSDF-1-β (human Stromal Cell-Derived Factor-1-beta).
• Antibody Type: Primary Antibody
• Concentration: batch dependent
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• Target: CXCL12
• UniProt ID: P48061
• NCBI: P48061
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: IRH, PBSF, SDF1, TLSF, TPAR1, SCYB12, SDF1A, SDF1B
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

To detect Human SDF-1-beta by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Anti-Human SDF-1-beta (orb1272311) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Human SDF-1-beta.

To detect Human SDF-1-beta by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Human SDF-1-beta is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

To detect Human SDF-1-beta by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Human SDF-1-beta is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

This antibody stained formalin-fixed, paraffin-embedded sections of human pancreas malignant adenocarcinoma. The recommended concentration is 2.5-5.0 ug/ml with an overnight incubation at 4°C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 9.0 buffer is recommended. Optimal concentrations and conditions may vary.