SDF-1a Antibody

• Catalog Number: orb1272316
• Category: Antibodies
• Description: SDF-1a Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, NeA, WB
• Reactivity: Human
• Immunogen: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hSDF-1-alpha (human Stromal Cell-Derived Factor-1-alpha).
• Antibody Type: Primary Antibody
• Concentration: batch dependent
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• Target: CXCL12
• UniProt ID: P48061
• NCBI: P48061
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: IRH, PBSF, SDF1, TLSF, TPAR1, SCYB12, SDF1A, SDF1B
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

To detect Human SDF-1-alpha by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Anti-Human SDF-1-alpha (orb1272315) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Human SDF-1-alpha.

To detect Human SDF-1-alpha by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Human SDF-1-alpha is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

To detect Human SDF-1-alpha by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Human SDF-1-alpha is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

This antibody stained formalin-fixed, paraffin-embedded sections of human colorectal adenocarcinoma. The recommended concentration is 0.5 ug/ml with an overnight incubation at 4°C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6.0 sodium citrate buffer is recommended. Optimal concentrations and conditions may vary.