SARS-CoV-2 Spike P26S Antibody (Gamma Variant)

• Catalog Number: orb1239024
• Category: Antibodies
• Description: SARS-CoV-2 Spike P26S Antibody (Gamma Variant)
• Target: S
• Clonality: Polyclonal
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Virus
• Form/Appearance: Liquid
• Concentration: 1 mg/mL
• Buffer/Preservatives: SARS-CoV-2 Spike P26S antibody is supplied in PBS containing 0.02% sodium azide.
• Purification: SARS-CoV-2 Spike P26S (Gamma Variant) antibody is affinity chromatography purified via peptide column.
• Immunogen: Anti-SARS-CoV-2 Spike P26S (Gamma Variant) antibody (orb1239024) was raised against a peptide corresponding to 11 amino acids near the amino terminus of SARS-CoV-2 Gamma variant (P.1) Spike protein. The immunogen is located within the first 50 amino acids of SARS-CoV-2 Gamma variant (P.1) Spike protein.
• UniProt ID: P0DTC2
• Tested applications: ELISA, WB
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: SARS-CoV-2 (COVID-19) Spike Antibody: Severe acute
• Research Area: Infectious Disease & Virology
• Note: For research use only
• NCBI: QHD43416

SARS-Cov-2 Spike P26S (Gamma Variant) Antibodies Specifically Detect Gamma Variant Spike S1 Protein in an ELISA. Coating Antigen: SARS-CoV-2 spike S1 proteins WT, alpha variant (B.1.1.7), beta variant (B.1.351), gamma variant (P.1), delta variant (B.1.617.2), mu variant (B.1.621), and omicron variant (B.1.1.529), 1 µg/mL, incubate at 4 ˚C overnight. Detection Antibodies: SARS-CoV-2 Spike P26S (Gamma Variant) antibody, orb1239024, dilution: 200 and 1000 ng/mL, incubate at RT for 1 hr. Secondary Antibodies: Goat anti-rabbit HRP at 1:20000, incubate at RT for 1 hr.

ELISA Validation of Gamma Variant Spike S1 Antibodies with SARS-CoV-2 Gamma Variant Spike S1 Protein. Coating Antigen: SARS-CoV-2 spike S1 proteins WT and Gamma variant (P.1), 1 µg/mL, incubate at 4 ˚C overnight. Detection Antibodies: SARS-CoV-2 Spike P26S (Gamma Variant) antibody, orb1239024, dilution: 0.3-1000 ng/mL, incubate at RT for 1 hr. Secondary Antibodies: Goat anti-rabbit HRP at 1:20000, incubate at RT for 1 hr. SARS-CoV-2 Spike P26S (Gamma Variant) antibody (orb1239024) specifically detects Gamma variant spike S1 protein, but not WT spike S1 protein (orb1231589).

WB Validation of Gamma Variant Spike S1 Antibodies with SARS-CoV-2 Gamma Variant Spike S1 Protein. Loading: 50 ng of SARS-CoV-2 spike S1 proteins, including WT and Gamma variant (P.1). Detection Antibodies: Spike P26S (Gamma Variant) antibody, orb1239024, 1 µg/mL, incubate at RT for 1 hr. Secondary Antibodies: Goat anti-rabbit HRP at 1:20000, incubate at RT for 1 hr. SARS-CoV-2 Spike P26S (Gamma Variant) antibody (orb1239024) specifically detects Gamma variant spike S1 protein, but not WT spike S1 protein (orb1231589).