SARS-CoV-2 (COVID-19) Spike S1 Antibody (biotin)

• Catalog Number: orb1239026
• Category: Antibodies
• Description: SARS-CoV-2 (COVID-19) Spike S1 Antibody (biotin)
• Target: S
• Clonality: Polyclonal
• Isotype: IgG
• Conjugation: Biotin
• Reactivity: Virus
• Form/Appearance: Liquid
• Concentration: 1 mg/mL
• Buffer/Preservatives: SARS-CoV-2 (COVID-19) Spike S1 Antibody (biotin) is supplied in PBS containing 0.02% sodium azide.
• Purification: SARS-CoV-2 (COVID-19) Spike S1 Antibody (biotin) is affinity chromatography purified via peptide column.
• Immunogen: Anti-SARS-CoV-2 (COVID-19) Spike S1 Antibody (biotin) (orb1239026) was raised against a peptide corresponding to 16 amino acids near the amino terminus of SARS-CoV-2 (COVID-19) Spike S1 glycoprotein. The immunogen is located within the first 50 amino acids of SARS-CoV-2 (COVID-19) Spike S1 protein.
• UniProt ID: P0DTC2
• Tested applications: ELISA
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: SARS-CoV-2 (COVID-19) Spike S1 Antibody (biotin):
• Research Area: Infectious Disease & Virology
• Note: For research use only
• NCBI: QHD43416
• Expiration Date: 12 months from date of receipt.

ELISA Validation of SARS-CoV-2 Spike Antibody Pair.A sandwich ELISA was performed using the anti-SARS-COV-2 Spike S1 antibodies orb1239981 (10 μg/mL) as the capture antibody. Biotin-labled anti-SARS-COV-2 Spike S1 antibodies orb1239026 (1 μg/mL) and streptavidin-HRP (0.1 μg/mL) were used for detection. Detection range is from 0.8 ng to 200 ng (SARS-CoV-2 Spike S1 Protein, orb1227612).

Sandwich ELISA for SARS-CoV-2 (COVID-19) Matched Pair Spike S1 Antibodies. Antibodies: SARS-CoV-2 (COVID-19) Spike Antibodies, orb1274178 and orb1239026. A sandwich ELISA was performed using SARS-CoV-2 Spike S1 antibody (orb1274178, 2ug/ml) as capture antibody, the Spike S1 recombinant protein as the binding protein (orb1227612), and the anti-SARS-CoV-2 Spike S1 antibody (orb1239026, 1ug/ml) as the detection antibody. Secondary: Streptavidin-HRP at 1:10000 dilution. Detection range is from 0.03 ng to 300 ng. EC50 = 5.1 ng

Sandwich ELISA for SARS-CoV-2 (COVID-19) Matched Pair Spike S1 Antibodies. Antibodies: SARS-CoV-2 (COVID-19) Spike Antibodies, orb1274177 and orb1239026. A sandwich ELISA was performed using SARS-CoV-2 Spike S1 antibody (orb1274177, 2ug/ml) as capture antibody, the Spike S1 recombinant protein as the binding protein (orb1227612), and the anti-SARS-CoV-2 Spike S1 antibody (orb1239026, 1ug/ml) as the detection antibody. Secondary: Streptavidin-HRP at 1:10000 dilution. Detection range is from 0.03 ng to 300 ng. EC50 = 1.96 ng