SARS-CoV-2 (COVID-19) Spike Antibody (HRP)

• Catalog Number: orb1239044
• Category: Antibodies
• Description: SARS-CoV-2 (COVID-19) Spike Antibody (HRP)
• Target: S
• Clonality: Polyclonal
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Virus
• Form/Appearance: Liquid
• Concentration: 1 mg/mL
• Buffer/Preservatives: SARS-CoV-2 (COVID-19) Spike Antibody is supplied in PBS containing 0.02% sodium azide.
• Purification: SARS-CoV-2 (COVID-19) Spike Antibody is affinity chromatography purified via peptide column.
• Immunogen: Anti-SARS-CoV-2 (COVID-19) Spike antibody (HRP) (3525) was raised against a peptide corresponding to 20 amino acids near the carboxy terminus of SARS-CoV-2 (COVID-19) Spike glycoprotein. The immunogen is located within the last 50 amino acids of SARS-CoV-2 (COVID-19) Spike protein.
• UniProt ID: P0DTC2
• Tested applications: ELISA, IHC
• Application notes: IHC: 1 μg/mL Antibody validated: Immunohistochemistry in COVID-19 patient samples. All other applications and species not yet tested.
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: SARS-CoV-2 (COVID-19) Spike Antibody: Severe acute
• Note: For research use only
• NCBI: QHD43416

Immunohistochemistry Validation of SARS-CoV-2 (COVID-19) Spike in COVID-19 Patient Lung. Immunohistochemical analysis of paraffin-embedded COVID-19 patient lung tissue using anti- SARS-CoV-2 (COVID-19) spike antibody (orb1239044, 2 ug/mL). Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4 °C. Counter stained with Hematoxylin. Strong signal of SARS-COV-2 spike protein was observed in macrophages of COVID-19 patient lung, but not in non-COVID-19 patient lung.

ELISA Validation. Coating Antigen: immunogen peptide, orb1236596, 10 ug/mL, incubate at 4 °C overnight. Detection Antibodies: SARS-CoV-2 Spike antibody, orb1239044 or orb1239976, dilution: 0.5-1000 ng/mL, incubate at RT for 1 hr. orb1239976 was detected by anti-rabbit HRP-conjugated secondary antibodies at 1:10000, incubate at RT for 1 hr.