SARS-CoV-2 (COVID-19) ORF9c Antibody

• Catalog Number: orb1239056
• Category: Antibodies
• Description: SARS-CoV-2 (COVID-19) ORF9c Antibody
• Target: ORF9c
• Clonality: Polyclonal
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Virus
• Form/Appearance: Liquid
• Concentration: 1 mg/mL
• Buffer/Preservatives: SARS-CoV-2 (COVID-19) ORF9c antibody is supplied in PBS containing 0.02% sodium azide.
• Purification: SARS-CoV-2 (COVID-19) ORF9c Antibody is affinity chromatography purified via peptide column.
• Immunogen: Anti-SARS-CoV-2 (COVID-19) ORF9c antibody (orb1239056) was raised against a peptide corresponding to 16 amino acids near the amino-terminus of SARS-CoV-2 (COVID-19) ORF9c protein. The immunogen is located within the first 50 amino acids of the SARS-CoV-2 (COVID-19) ORF9c protein.
• UniProt ID: P0DTD3
• MW: 8kD
• Tested applications: ELISA, WB
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: ORF9c protein, Uncharacterized protein 14, ORF14,
• Research Area: Infectious Disease & Virology
• Note: For research use only
• NCBI: P0DTD3
• Expiration Date: 12 months from date of receipt.

Western Blot Validation with SARS-CoV-2 (COVID-19) ORF9c Overexpressed 293 Cells. Loading: 10 µg per lane of 293 cell lysate from WT and SARS-CoV-2 (COVID-19) ORF9c transfected cells. Antibodies: SARS-CoV-2 (COVID-19) ORF9c orb1239056, 1 µg/mL, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Western Blot Validation with SARS-CoV-2 (COVID-19) ORF9c Overexpressed 293 Cells. Loading: 10 µg per lane of 293 cell lysate from WT and SARS-CoV-2 (COVID-19) ORF9c transfected cells. Antibodies: SARS-CoV-2 (COVID-19) ORF9c, orb1239056, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. Lane 1: 0.25 µg/mL and. Lane 2: 0.5 µg/mL.

ELISA Validation. Antibodies: SARS-CoV-2 (COVID-19) ORF9c Antibody, orb1239056. A direct ELISA was performed using SARS-CoV-2 ORF9c immunogen peptide (orb1231666) as coating antigen and the anti-SARS-CoV-2 (COVID-19) ORF9c antibody as the capture antibody. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:20000 dilution. Detection range is from 0.3 ng/mL to 1000 ng/mL.