SARS-CoV-2 (COVID-19) Nucleocapsid Antibody [84C4a]

• Catalog Number: orb1274607
• Category: Antibodies
• Description: SARS-CoV-2 (COVID-19) Nucleocapsid Antibody [84C4a]
• Target: N
• Clonality: Recombinant
• Isotype: IgG1k
• Conjugation: Unconjugated
• Reactivity: Virus
• Form/Appearance: Liquid
• Concentration: batch dependent
• Buffer/Preservatives: 1X PBS, 0.02% NaN3, 50% Glycerol, 0.1% rAlbumin
• Purification: SARS-CoV-2 (COVID-19) Nucleoprotein Antibody [84C4a] is affinity chromatography purified via peptide column.
• Immunogen: SARS-CoV-2 nucleoprotein (NP) specific peptide
• Tested applications: ELISA
• Application notes: Paired with 75G5a, used for specific, high-sensitivity detection of SARS-CoV-2 nucleocapsid protein (NP).
• Antibody Type: Primary Antibody
• Clone Number: 84C4a
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: SARS-CoV-2 (COVID-19) Nucleoprotein Antibody [P002
• Research Area: Infectious Disease & Virology
• Note: For research use only
• NCBI: QHD43423
• Expiration Date: 12 months from date of receipt.

Microtiter wells were coated with SARS-CoV-2 (COVID-19) Nucleocapsid Protein (NP) and SARS-CoV NP at 1 ug/mL. Purified rabbit monoclonal antibody was serially diluted 1:2 starting at 1 ug/mL, and shows very strong and specific binding to COVID-19 NP antigen, with no significant cross-reactivity to SARS-CoV NP antigen.

Immunohistochemistry Validation of SARS-CoV-2 (COVID-19) Nucleocapsid in COVID-19 Patient Lung. Immunohistochemical analysis of paraffin-embedded COVID-19 patient lung tissue using anti-SARS-CoV-2 (COVID-19) Nucleocapsid antibody (orb1274607, 5 ng/mL). Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4 ˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin. Strong signal of SARS-COV-2 Nucleocapsid protein was observed in the macrophages of COVID-19 patient lung, but not in non-COVID-19 patient lung.

A sandwich ELISA was performed using SARS-CoV-2 (COVID-19) Nucleocapsid Protein (NP) specific rabbit monoclonal antibodies. COVID-19 NP was serially diluted 1:2 starting at 25 ng/mL.

Western blot analysis of SARS-CoV2 and SARS-CoV nucleocapsid protein (50 ng) probed with 1 ug/mL CoV2NP rabbit monoclonal antibody.