SARS-CoV-2 (COVID-19) Nucleocapsid Antibody [3868]

• Catalog Number: orb1273019
• Category: Antibodies
• Description: SARS-CoV-2 (COVID-19) Nucleocapsid Antibody [3868]
• Clonality: Monoclonal
• Isotype: Mouse IgG1
• Conjugation: Unconjugated
• Reactivity: Virus
• Form/Appearance: Liquid
• Concentration: batch dependent
• Buffer/Preservatives: Coronavirus Nucleocapsid Antibody is in a phosphate saline buffer (0.01M, pH 7.2) containing 0.1% sodium azide preservative. No stabilizing proteins have been added.
• Purification: Coronavirus Nucleocapsid Antibody is purified from ascites fluid or culture medium by protein A chromoatography or sequential differential precipitations.
• Immunogen: These newer COVID-19 nucleoprotein Mabs were developed using a recombinant, full-length nucleoprotein and then confirmed that they are reactive with the native virus itself.
• Tested applications: ELISA, IF
• Application notes: Suggested starting ranges are 1:10-1:50 for IFA and 1:20-1:200 for ELISA. BEST ELISA pairs (capture-conjugate): clones 3865-3868 / 3869-3868
• Antibody Type: Primary Antibody
• Clone Number: 3868
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Coronavirus Nucleocapsid, SARS
• Note: For research use only

Sandwich ELISA for SARS-CoV-2 (COVID-19) Matched Pair Nucleocapsid Antibodies. Antibodies: SARS-CoV-2 (COVID-19) Nucleocapsid Antibodies, orb1274588 and orb1273022. A sandwich ELISA was performed using SARS-CoV-2 Nucleocapsid antibody (orb1274588, 2 ug/ml) as capture antibody, the Nucleocapsid recombinant protein as the binding protein (orb1231585), and the anti-SARS-CoV-2 Nucleocapsid antibody (orb1273022, 0.5 ug/ml) as the detection antibody. Secondary: Goat anti-mouse IgG HRP conjugate at 1:20000 dilution. Detection range is from 0.03 ng to 300 ng. EC50 = 15.82 ng

Sandwich ELISA for SARS-CoV-2 (COVID-19) Matched Pair Nucleocapsid Antibodies. Antibodies: SARS-CoV-2 (COVID-19) Nucleocapsid Antibodies, orb1274589 and orb1273019. A sandwich ELISA was performed using SARS-CoV-2 Nucleocapsid antibody (orb1274589, 2 ug/ml) as capture antibody, the Nucleocapsid recombinant protein as the binding protein (orb1231585), and the anti-SARS-CoV-2 Nucleocapsid antibody (orb1273019, 0.5 ug/ml) as the detection antibody. Secondary: Goat anti-mouse IgG HRP conjugate at 1:20000 dilution. Detection range is from 0.03 ng to 300 ng. EC50 = 4.27 ng