SARS-CoV-2 (COVID-19) NSP13 (Helicase) Antibody

• Catalog Number: orb1239079
• Category: Antibodies
• Description: SARS-CoV-2 (COVID-19) NSP13 (Helicase) Antibody
• Target: HEL
• Clonality: Polyclonal
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Virus
• Form/Appearance: Liquid
• Concentration: 1 mg/mL
• Buffer/Preservatives: SARS-CoV-2 (COVID-19) NSP13 (Helicase) Antibody is supplied in PBS containing 0.02% sodium azide.
• Purification: SARS-CoV-2 (COVID-19) NSP13 (Helicase) Antibody is affinity chromatography purified via peptide column.
• Immunogen: Anti-SARS-CoV-2 (COVID-19) NSP13 (Helicase) antibody (orb1239079) was raised against a peptide corresponding to 19 amino acids near the carboxyl terminus of the SARS-CoV-2 (COVID-19) NSP13 (Helicase) protein. The immunogen is located in the last 50 amino acids of SARS-CoV-2 (COVID-19) NSP13 (Helicase).
• UniProt ID: P0DTC1
• Tested applications: ELISA, IHC
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Helicase, Non-structural protein 13, NSP13
• Research Area: Infectious Disease & Virology
• Note: For research use only
• NCBI: YP_009725308.1
• Expiration Date: 12 months from date of receipt.

Immunohistochemistry Validation of SARS-CoV-2 (COVID-19) NSP13 in COVID-19 Patient Lung. Immunohistochemical analysis of paraffin-embedded COVID-19 patient lung tissue using anti-SARS-CoV-2 (COVID-19) NSP13 antibody (orb1239079, 0.5 µg/mL). Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4 ˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin. Strong signal of SARS-COV-2 NSP13 protein was observed in macrophage of COVID-19 patient lung, but not in non-COVID-19 patient lung.

ELISA Validation with SARS-CoV-2 (COVID-19) NSP13 (Helicase) Protein. Antibodies: SARS-CoV-2 (COVID-19) NSP13 (Helicase) Antibody, orb1239079. A direct ELISA was performed using SARS-CoV-2 NSP13 recombinant protein (orb1231563) as coating antigen and the anti-SARS-CoV-2 (COVID-19) NSP13 (Helicase) antibody as the capture antibody. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:20000 dilution. Detection range is from 1 ng/mL to 3000 ng/mL.

ELISA Validation. Antibodies: SARS-CoV-2 (COVID-19) NSP13 (Helicase) Antibody, orb1239079. A direct ELISA was performed using immunogen peptide (orb1231680) as coating antigen and the anti-SARS-CoV-2 (COVID-19) NSP13 (Helicase) antibody as the capture antibody. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:20000 dilution. Detection range is from 0.3 ng/mL to 1000 ng/mL.