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RecombinantIGF-II,Human
Catalog Number: orb1494836
| Catalog Number | orb1494836 |
|---|---|
| Category | Proteins |
| Description | Insulin-like Growth Factor II (IGF-II) is a single chain 7 kDa polypeptide, and shares a high degree of homology with insulin. During circulation in vivo, IGF-II is complexed to high affinity binding proteins, IGF Binding Proteins (IGFBP), which act as circulating reservoirs, transport IGF-II, and prolong the half life of IGF-II. The receptors of IGF-II (IGFRs) are transmembrane tyrosine receptors, and are heterotetrameric consisting of two α-subunits and two β-subunits. IGFRs execute their role via intracellullar signaling molecules, such as IRS, shc, and PI3K. The functions of IGF-II include promoting cell survival, growth, proliferation, differentiation and motility. In particular, IGF-II promotes proliferation, inhibits death, and stimulates transformation in breast cancer cells.Recombinant human Insulin-like Growth Factor II (rhIGF-II) produced in E.coli is a single non-glycosylated polypeptide chain containing 68 amino acids. A fully biologically active molecule, rhIGF-II has a molecular mass of 7.6 kDa analyzed by reducing SDS-PAGE and is obtained by proprietary chromatographic techniques at GenScript. |
| Form/Appearance | Lyophilized after extensive dialysis against 25mM Tris, pH 8.0. |
| Purity | > 95% as analyzed by SDS-PAGE and HPLC. |
| MW | 7.6 kDa, observed by reducing SDS-PAGE. |
| Protein Sequence | MAYRPSETLCG GELVDTLQFV CGDRGFYFSR PASRVSRRSR GIVEECCFRS CDLALLETYC ATPAKSE |
| Source | Escherichia coli. |
| Biological Activity | ED50 5×10ˆ4 units/mg. |
| Endotoxins | < 0.2 EU/μg, determined by LAL method. |
| Storage | Lyophilized recombinant human Insulin-like Growth Factor II (rhIGF-II) remains stable up to 6 months at -80°C from date of receipt. Upon reconstitution, rhIGF-II remains stable up to 2 weeks at 4°C or up to 3 months at -20°C. |
| Buffer/Preservatives | Lyophilized after extensive dialysis against 25mM Tris, pH 8.0. |
| Alternative names | Insulin-like growth factor II Read more... |
| Note | For research use only |
| Application notes | Reconstituted in ddH2O at 100 μg/mL. |
| Expiration Date | 6 months from date of receipt. |
