Recombinant human TNF alpha Antibody

• Catalog Number: orb2657852
• Category: Antibodies
• Description: Recombinant human TNF alpha Antibody
• Clonality: Recombinant
• Conjugation: Unconjugated
• Purification: Purified by affinity chromatography. Lyophilized from a 0.2 μm filtered solution in Storage buffer (100 mM Tris, 150 mM NaCl, 0.01% Tween 20, pH 8.0).
• Tested applications: Functional Studies
• Application notes: Recombinant protein TNF alpha is expressed in S2 cell (Drosophila). DNA sequence encoding 157 AAs was fused with Strep-tag at the N-terminus. A calculated molecular mass of recombinant protein is 18.7 kDa.
• Antibody Type: Recombinant Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Note: For research use only
• Entrez: 35
• Expiration Date: 12 months from date of receipt.

Recombinant Human TNF alpha induces cytotoxicity in HeLa cell line in the presence of the protein synthesis inhibitor cycloheximide. Measured in WST-8 fluorometric assay method. The ED50 for this effect is 4.2513 ng/mL.

Recombinant Human TNF alpha induces cytotoxicity in HeLa cell line in the presence of the protein synthesis inhibitor cycloheximide. Measured in WST-8 fluorometric assay method. The ED50 for this effect is 4.2513 ng/mL.

Recombinant Human TNF alpha induces cytotoxicity in HeLa cell line in the presence of the protein synthesis inhibitor cycloheximide. Measured in WST-8 fluorometric assay method. The ED50 for this effect is 4.2513 ng/mL.