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Catalog Number | orb1516367 |
---|---|
Category | Proteins |
Description | Recombinant human Noggin (HEK293) |
Tag | Tag free |
Concentration | >0.5 mg/ml |
Form/Appearance | Lyophilized |
Purity | > 95% as determined by Tris-Bis PAGE |
MW | Due to glycosylation, the protein migrates to 30-36 kDa based on Tris-Bis PAGE result. |
Target | Noggin |
Protein Sequence | 28-232/232 |
Expression System | HEK293 cell |
Biological Activity | Not tested |
Endotoxins | < 1.0 EU/μg as determined by LAL |
Storage | Stored at -70°C or -20°C. Avoid repeated freeze/thaw cycles. |
Buffer/Preservatives | Lyophilized from 0.22um filtered solution in PBS (pH7.4) with 5mM DTT. Normally 5% trehalose is added as protectant before Lyophilization. |
Alternative names | NOG; SYM 1; SYM1; Symphalangism 1 (proximal); Syno Read more... |
Note | For research use only |
Application notes | Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Expiration Date | 6 months from date of receipt. |
Recombinant Human Noggin Protein on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
> 90% as determined by SDS PAGE | |
23 kDa |
98.00% | |
25.16 kDa (predicted). Due to glycosylation, the protein migrates to 28-38 kDa based on Tris-Bis PAGE result. |
> 95% | |
23.07 kDa (predicted). Due to glycosylation, the protein migrates to 28-33 kDa based on Tris-Bis PAGE result. |
96.80% | |
49.8 kDa (predicted); 58-62 kDa (reducing condition, due to glycosylation) |
SDS-PAGE: 99.1%; SEC-HPLC: 99.3% | |
23.1 kDa (predicted); 30 kDa (reducing condition, due to glycosylation) |