Recombinant Human Interferon alpha-2 (IFNA2) (Active)

• Catalog Number: orb2659094
• Category: Proteins
• Description: Recombinant Human Interferon alpha-2 (IFNA2) (Active)
• Tag: N-terminal 10xHis-tagged
• Form/Appearance: Lyophilized powder
• Purity: Greater than 95% as determined by SDS-PAGE.
• MW: 22.9 kDa
• UniProt ID: P01563
• Protein Sequence: CDLPQTHSLGSRRTLMLLAQMRRISLFSCLKDRHDFGFPQEEFGNQFQKAETIPVLHEMIQQIFNLFSTKDSSAAWDETLLDKFYTELYQQLNDLEACVIQGVGVTETPLMKEDSILAVRKYFQRITLYLKEKKYSPCAWEVVRAEIMRSFSLSTNLQESLRSKE
• Protein Length: Full Length of Mature Protein
• Source: Mammalian cell
• Biological Origin: Homo sapiens (Human)
• Biological Activity: ①Measured by its binding ability in a functional ELISA. Immobilized Human IFNA2 at 2 μg/mL can bind Human IFNAR2(CSB-MP011047HU). The EC50 is 154.2-191.9 ng/mL. ②Measured by its binding ability in a functional ELISA. Immobilized Human IFNA2 at 2 μg/mL can bind Anti-IFNA2 recombinant antibody(CSB-RA365593MA1HU). The EC50 is 2.366-2.818 ng/mL.
• Expression Region: 24-188aa
• Endotoxins: Less than 1.0 EU/ug as determined by LAL method.
• Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
• Buffer/Preservatives: Lyophilized from a 0.2 μm sterile filtered PBS, 6% Trehalose, pH 7.4
• Alternative names: IFN-alpha-2; Interferon alpha-A (LeIF A 1)
• Note: For research use only
• Application notes: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
• Expiration Date: 6 months from date of receipt.

Measured by its binding ability in a functional ELISA. Immobilized Human IFNA2 at 2 μg/ml can bind Human IFNAR2. The EC50 is 154.2-191.9 ng/mL.

Measured by its binding ability in a functional ELISA. Immobilized Human IFNA2 at 2 μg/ml can bind Anti-IFNA2 recombinant antibody. The EC50 is 2.366-2.818 ng/mL.

(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.