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Item 1 of 3
Recombinant human B7-H3/CD276 protein, C-His (HEK293)
Catalog Number: orb1516515
| Catalog Number | orb1516515 |
|---|---|
| Category | Proteins |
| Description | Recombinant human B7-H3/CD276 protein, C-His (HEK293) |
| Target | CD276 antigen |
| Tag | C-His |
| Form/Appearance | Lyophilized |
| Concentration | ＞0.5 mg/ml |
| Buffer/Preservatives | Lyophilized from 0.22um filtered solution in PBS (pH7.4) with 5mM DTT. Normally 5% trehalose is added as protectant before Lyophilization. |
| Purity | > 95% as determined by Tris-Bis PAGE; > 95% as determined by SEC-HPLC |
| Protein Sequence | 29-245/534 |
| MW | Due to glycosylation, the protein migrates to 40-50 kDa based on Tris-Bis PAGE result. |
| Application notes | Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. |
| Expression System | HEK293 cell |
| Endotoxins | < 1.0 EU/μg as determined by LAL |
| Biological Activity | Not tested |
| Storage | Stored at -70°C or -20°C. Avoid repeated freeze/thaw cycles. |
| Alternative names | B7H3; 4Ig B7 H3; 4Ig-B7-H3; AU016588; B7 H3; B7 ho Read more... |
| Note | For research use only |
Human B7-H3 / CD276 Protein on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Immobilized Human B7-H3, His Tag at 0.5 µg/ml (100 µl/Well) on the plate. Dose response curve for Anti-Human B7-H3 with the EC50 of 24.8 ng/ml determined by ELISA.
The purity of Human B7-H3 / CD276 Protein is greater than 95% as determined by SEC-HPLC.
