Recombinant CD59 Antibody

• Catalog Number: orb2635673
• Category: Antibodies
• Description: CD59 regulates complement-mediated cell lysis and it is involved in lymphocyte signal transduction.
• Clonality: Recombinant
• Species/Host: Rabbit
• Isotype: Rabbit IgG, kappa
• Conjugation: Unconjugated
• Reactivity: Human
• Immunogen: Recombinant human full-length CD59 protein was used as the immunogen for the recombinant CD59 antibody.
• UniProt ID: P13987
• Tested applications: IHC-P
• Dilution range: Immunohistochemistry (FFPE): 1-2ug/ml
• Application notes: Optimal dilution of the recombinant CD59 antibody should be determined by the researcher.
• Antibody Type: Primary Antibody
• Clone Number: MACIF/7021R
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

IHC staining of FFPE human spleen tissue with recombinant CD59 antibody (clone MACIF/7021R). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human tonsil tissue with recombinant CD59 antibody (clone MACIF/7021R). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human kidney tissue with recombinant CD59 antibody (clone MACIF/7021R) at 2 ug/ml. HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human cerebellum tissue with recombinant CD59 antibody (clone MACIF/7021R) at 2 ug/ml. Cytoplasmic and membranous staining observed. Negative control inset: PBS used instead of primary antibody to control for secondary Ab binding. HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

SDS-PAGE analysis of purified, BSA-free recombinant CD59 antibody (clone MACIF/7021R) as confirmation of integrity and purity.