Recombinant Bcl-X (Apoptosis Marker) Antibody

• Catalog Number: orb1410448
• Category: Antibodies
• Description: Mouse monoclonal antibody to Bcl-X (Apoptosis Marker)
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: rBCL2L1/4508
• Tested applications: IHC
• Reactivity: Human
• Isotype: IgG2a
• Immunogen: Recombinant fragment (around aa1-100) of human BCL2L1 protein (exact sequence is proprietary)
• Antibody Type: Recombinant Antibody
• Concentration: Purified Ab with BSA and Azide at 200ug/ml
• Dilution range: Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.
• Form/Appearance: 200ug/ml of Ab produced in CHO cell mammalian-based expression system. Prepared in 10mM PBS with 0.05% rAlbumin & 0.05% azide. Also available WITHOUT rAlbumin & azide at 1.0mg/ml.
• Conjugation: Unconjugated
• MW: 27kDa
• Target: BCL2L1
• Entrez: 598
• UniProt ID: Q07817
• Expression System: CHO cells
• Storage: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
• Buffer/Preservatives: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% rAlbumin & 0.05% azide. Also available WITHOUT rAlbumin & azide at 1.0mg/ml.
• Alternative names: Apoptosis regulator Bcl-X, B cell lymphoma 2 like,
• Note: For research use only
• Application notes: Positive Control: Jurkat, K562, HL-60 or HeLa cells. Reed-Sternberg cells in Hodgkin s lymphoma.
• Expiration Date: 12 months from date of receipt.